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MASSONS TRICHROME STAIN PRESENTED BY: MEHTAB SHAH  B.S MT 3 Rd  year  (SDLS(LNH) HISTOPATHOLOGY
SPECIAL STAINING ,[object Object]
INTRODUCTION ,[object Object],[object Object],[object Object]
Muscle ,[object Object],[object Object],[object Object],[object Object]
SMOOTH MUSCLE (involuntary) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
COLLAGEN ,[object Object],[object Object],[object Object],[object Object],[object Object]
Contd …………….. ,[object Object]
PRINCIPLE ,[object Object],[object Object],[object Object],[object Object]
Contd……………………. ,[object Object],[object Object],[object Object]
FIXATIVE   Bouin’s solution is preferred 10% neutral buffered formalin may be used TECHNIQUE Cut paraffin section at 4 microns
REAGENT REQUIRMENT ,[object Object],[object Object],[object Object],[object Object],[object Object]
PROCEDURE ,[object Object],[object Object],[object Object],[object Object],[object Object]
Contd……………………… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Light green solution 2-5mint; Dehydrate in absolute alcohol Clear in xylene Mount in synthetic resin Microscopy
Quality control and safety ,[object Object],[object Object]
Results ,[object Object],[object Object],[object Object]
 
Nuclei red, collagen green, cytoplasm red
Infracted rat heart showing damaged cardiac tissue in blue.
Terminal ileum with increase in subepithelial collagen table in a patient with collagenous colitis
 

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Massons Trichrome Stain

  • 1.  
  • 2. MASSONS TRICHROME STAIN PRESENTED BY: MEHTAB SHAH B.S MT 3 Rd year (SDLS(LNH) HISTOPATHOLOGY
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11. FIXATIVE Bouin’s solution is preferred 10% neutral buffered formalin may be used TECHNIQUE Cut paraffin section at 4 microns
  • 12.
  • 13.
  • 14.
  • 15. Light green solution 2-5mint; Dehydrate in absolute alcohol Clear in xylene Mount in synthetic resin Microscopy
  • 16.
  • 17.
  • 18.  
  • 19. Nuclei red, collagen green, cytoplasm red
  • 20. Infracted rat heart showing damaged cardiac tissue in blue.
  • 21. Terminal ileum with increase in subepithelial collagen table in a patient with collagenous colitis
  • 22.