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Hello this is a nice assignment and some
pictures on chromatography
• Made by han buwalda luzac lyceum the hague
• My second work: hanbuwalda.teamasea.com
is on a breakthrough (nobelprice equivalent) on cellullar health
CHROMATOGRAPHY
Principles and images
Composed by Han Buwalda @ LinkedIn
Luzac Lyeum 070
Hello Tiger Education
hanbuwalda.teamasea.com
Paper chromatography
• Most known by separating the colours
out of markers
• You draw a point just above the
level of the liquid (your solvent
in NL ‘loopvloeistof’//running liquid)
• Due to the ratio of adsorbance and solubility the colours (= distinguishable
compounds) will separate on ‘climbing up’.
• wiki/Papierchromatografie
• Please try this at home using different
markers and a bit of diluted vinegar
Assignment
• What types of chromatography are available?
• What are the key elements? Make a list individually
• Compare the different methods.
• What do they have in common? Compare together
• Do you all understand it all?
• Make another list of the shared key elements/principles .
The last list contains the principles of the technique to analyse ….?
• Include pictures!!!! Talk about it.
• You can do this assignment using the expert method.
• Eg 4 students each study one technique and share their results and then using a
chairman make a list of the chromatography features.
• Eventually each student should be able to apply this knowledge in an unknown situation.
Liquid chromatography
• We take high pressure LC = HPLC
• The key to success is to vary
• The solvent
• The column
Thin layer chromatography = TLC
From a Dutch assignment (K9 or class HV3)
• In dit verslag over het scheiden van de kleurstoffen van de
verschillende stiften behandel je theorie en doel van chromatografie
• Voor de theorie maak je gebruik van de twee belangrijke begrippen:
de zwemmers (de kleurstoffen uit een stift die ver komen) en de
plakkers (de kleurstoffen die niet ver komen)
• Je koppelt deze twee begrippen aan de goede vaktermen.

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Chromatography 6 v k12 in english hb ll 070

  • 1. Hello this is a nice assignment and some pictures on chromatography • Made by han buwalda luzac lyceum the hague • My second work: hanbuwalda.teamasea.com is on a breakthrough (nobelprice equivalent) on cellullar health
  • 2. CHROMATOGRAPHY Principles and images Composed by Han Buwalda @ LinkedIn Luzac Lyeum 070 Hello Tiger Education hanbuwalda.teamasea.com
  • 3. Paper chromatography • Most known by separating the colours out of markers • You draw a point just above the level of the liquid (your solvent in NL ‘loopvloeistof’//running liquid) • Due to the ratio of adsorbance and solubility the colours (= distinguishable compounds) will separate on ‘climbing up’. • wiki/Papierchromatografie • Please try this at home using different markers and a bit of diluted vinegar
  • 4. Assignment • What types of chromatography are available? • What are the key elements? Make a list individually • Compare the different methods. • What do they have in common? Compare together • Do you all understand it all? • Make another list of the shared key elements/principles . The last list contains the principles of the technique to analyse ….? • Include pictures!!!! Talk about it. • You can do this assignment using the expert method. • Eg 4 students each study one technique and share their results and then using a chairman make a list of the chromatography features. • Eventually each student should be able to apply this knowledge in an unknown situation.
  • 5. Liquid chromatography • We take high pressure LC = HPLC • The key to success is to vary • The solvent • The column
  • 7. From a Dutch assignment (K9 or class HV3) • In dit verslag over het scheiden van de kleurstoffen van de verschillende stiften behandel je theorie en doel van chromatografie • Voor de theorie maak je gebruik van de twee belangrijke begrippen: de zwemmers (de kleurstoffen uit een stift die ver komen) en de plakkers (de kleurstoffen die niet ver komen) • Je koppelt deze twee begrippen aan de goede vaktermen.