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090130_Journal_Club

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Andrew Hires
Andrew Hires

A review of photoactivatable transcription systems.

Technologie
1 von 19
Photoactivated Transcription 1/29/09 Andrew Hires Svoboda Journal Club
Photoactivated Transcription in Neurospora (Payen 1843) ,[object Object],[object Object]
Species Dependent Strategies ,[object Object],[object Object],[object Object],[object Object],[object Object]
Some Examples ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
phyR : Peak induction within 1 hour
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
phyR : Performance ,[object Object],50:1 LacZ induction, 1/5 expression of wild-type GAL4 system
phyR : Photon Dosing ,[object Object],1 minute pulse best 1mmol 660nm photons = 180 J 180 J / s m 2 = 180W / m 2 for 1s, or 3W / m 2 for 1 minute Filtered fluorescents for long term LEDs for pulsed activation
phyR : Temporal precision and reversibility ,[object Object],[object Object]
phyR : Issues ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
White Collar system
WC : Light activated transcription factor ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
WC : Light Responsive Element sequence
WC : wc-1 gene structure ,[object Object],[object Object],[object Object],[object Object],[object Object]
WC : FAD required for light sensitivity ,[object Object],[object Object],[object Object]
WC : Photon Dose and Wavelength ,[object Object],[object Object],[object Object]
WC : Two-photon action spectra ,[object Object],Heikal & Webb 2002
WC : Issues ,[object Object],[object Object],[object Object],[object Object],[object Object]

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