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By Taylor Cindric
What is DNA
Fingerprinting?

DNA Fingerprinting is a
way to identify a certain
individual, rather than
simply identifying a species
or a particular trait.
6 Steps to DNA Fingerprinting

 Step 1: Isolation of DNA
    DNA must be recovered
     from cells or tissue.
     Only a small amount of
     blood, hair, or skin is
     needed.
6 Steps to DNA Fingerprinting
                 Step 2: Cutting, Sizing, and
                  Sorting
                   Restriction enzymes are
                    used to cut the DNA at
                    specific places.
                   In a process called
                    electrophoresis, DNA
                    pieces are sorted out by
                    their size.
                   Then the DNA pieces are
                    passed through a gel made
                    of seaweed agarose.
6 Steps to DNA Fingerprinting
 Step 3: Transfer of
  DNA to Nylon
   The DNA pieces
    are transferred to
    a nylon sheet by
    placing the sheet
    on the gel and
    soaking them
    overnight.
6 Steps to DNA Fingerprinting
                 Step 4-5: Probing
                    Adding radioactive or
                     colored probes to the
                     nylon sheet produces a
                     pattern, which is the
                     DNA Fingerprint. Each
                     probe only sticks to one
                     or two specific places on
                     the sheet.
6 Steps to DNA Fingerprinting
 Step 6: DNA
 Fingerprint
   The final DNA
    fingerprint is built
    by using several
    probes. It
    resembles bar
    codes.
Uses of DNA Fingerprinting
               Diagnosis of Inherited Disorders
                 Helps diagnose disorders in
                  both prenatal and newborn
                  babies
                 Disorders may include cystic
                  fibrosis, hemophilia, Huntingto
                  n’s disease, familial
                  Alzheimer’s, sickle cell
                  anemia, thalassemia, and many
                  more.
Uses of DNA Fingerprinting
 Biological Evidence
    FBI and police labs
     around the country
     are starting to uses
     DNA fingerprinting
     to link suspects to
     biological evidence.
    Also it helps in the
     court system with
     paternity tests and
     child support.
Uses of DNA Fingerprinting
                Personal Identification
                   The U.S. armed services are
                    just beginning a program
                    where they collect DNA
                    fingerprints from all
                    personnel for later
                    use, incase they need to
                    identify casualties or
                    missing people.

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Dna fingerprinting

  • 2. What is DNA Fingerprinting? DNA Fingerprinting is a way to identify a certain individual, rather than simply identifying a species or a particular trait.
  • 3. 6 Steps to DNA Fingerprinting  Step 1: Isolation of DNA  DNA must be recovered from cells or tissue. Only a small amount of blood, hair, or skin is needed.
  • 4. 6 Steps to DNA Fingerprinting  Step 2: Cutting, Sizing, and Sorting  Restriction enzymes are used to cut the DNA at specific places.  In a process called electrophoresis, DNA pieces are sorted out by their size.  Then the DNA pieces are passed through a gel made of seaweed agarose.
  • 5. 6 Steps to DNA Fingerprinting  Step 3: Transfer of DNA to Nylon  The DNA pieces are transferred to a nylon sheet by placing the sheet on the gel and soaking them overnight.
  • 6. 6 Steps to DNA Fingerprinting  Step 4-5: Probing  Adding radioactive or colored probes to the nylon sheet produces a pattern, which is the DNA Fingerprint. Each probe only sticks to one or two specific places on the sheet.
  • 7. 6 Steps to DNA Fingerprinting  Step 6: DNA Fingerprint  The final DNA fingerprint is built by using several probes. It resembles bar codes.
  • 8. Uses of DNA Fingerprinting  Diagnosis of Inherited Disorders  Helps diagnose disorders in both prenatal and newborn babies  Disorders may include cystic fibrosis, hemophilia, Huntingto n’s disease, familial Alzheimer’s, sickle cell anemia, thalassemia, and many more.
  • 9. Uses of DNA Fingerprinting  Biological Evidence  FBI and police labs around the country are starting to uses DNA fingerprinting to link suspects to biological evidence.  Also it helps in the court system with paternity tests and child support.
  • 10. Uses of DNA Fingerprinting  Personal Identification  The U.S. armed services are just beginning a program where they collect DNA fingerprints from all personnel for later use, incase they need to identify casualties or missing people.